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صفحه اصلی
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سی و دومین کنفرانس بین المللی مهندسی برق
Modeling of dielectrophoretic single-stage continuous separation of Escherichia coli K38 in a microfluidic channel
نویسندگان :
Saeed Saedy
1
Navid Alaei Sheini
2
Shahrzad Ajabi
3
1- دانشگاه شهید چمران اهواز
2- دانشگاه شهید چمران اهواز
3- دانشگاه شهید چمران اهواز
کلمات کلیدی :
Escherichia coli،dielectrophoresis،K38،microfluidic channel،particle manipulation،label-free detection
چکیده :
Abstract— This study is focused on the use of dielectrophoretic (DEP) techniques in microfluidic channels to simulate the separation of Escherichia coli strain K38 (E. coli K38) without using labels for detection. The microfluidic channels used in the simulation were of dimensions 400 μm length and 50 μm width, with two inlets and two outlets having different velocities. The DEP manipulation was simulated at a frequency of 200 MHz and a voltage amplitude of approximately 3 Vpp. The study highlights the importance of detecting and separating bacteria in complex biological samples for various applications. Simulated microfluidic channels provide an ideal platform for studying and optimizing DEP-based techniques due to their ability to control fluid flow and simulate small sample volumes with great precision. The main objective of this study is to develop a robust and sensitive method for bacterial analysis by optimizing the DEP manipulation of E. coli K38 in simulated microfluidic channels. It was found that the displacement of the bacteria in the microfluidic channel was approximately 25 μm.
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